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Retatrutide

20MG Protocol

Retatrutide 20MG

Injection Freq.

Inject once weekly

Cycle Sched.

Minimum 24 weeks; trials extended to 48 weeks

Reconstitution

2.0 mL BAC water

Substantial weight loss reported in trials (up to ~24% at higher weekly doses)
HbA1c reductions (~2.0% reported in T2D trials at higher doses)
Improvements in lipids, blood pressure, and other cardiometabolic markers
Once-weekly dosing convenience
1

Reconstitution Requirements

  • 2.0 mL BAC water

Escalation steps: 4

Defined through week 12

StepWeek RangeDoseUnits
1Weeks 1–42 mg (2000 mcg)20
2Weeks 5–84 mg (4000 mcg)40
3Weeks 9–126 mg (6000 mcg)60
4Weeks 1+8 mg (8000 mcg)80

Inject once weekly

Minimum 24 weeks; trials extended to 48 weeks

Retatrutide (Retatrutide) is described as a first-in-class triple agonist that simultaneously activates GLP-1, GIP, and glucagon receptors to target weight and glucose physiology from three angles. The protocol explains the distinct role of each receptor: GLP-1 reduces appetite and slows gastric emptying; GIP enhances insulin secretion and may contribute to fat-metabolism effects; and glucagon receptor activation raises energy expenditure and promotes fat oxidation. This combination is presented as producing synergistic weight-loss and glycemic effects that exceed single- or dual-agonist incretin therapies. The page also notes engineering for duration—an approximately 6-day half-life supports once-weekly dosing. Clinical trial outcomes summarized include very large average body-weight reductions at higher weekly doses and meaningful HbA1c lowering in type 2 diabetes populations, alongside improvements in cardiometabolic markers. Overall, the mechanism is appetite reduction plus increased expenditure/fat oxidation, with GI side effects most common during dose escalation.
Nausea
Diarrhea
Vomiting
Constipation
Injection-site reactions (redness/swelling/discomfort)
  • Use aseptic technique: wipe vial stopper with alcohol; use new sterile syringe/needle
  • Add diluent slowly down the vial wall to minimize foaming
  • Gently swirl/roll until fully dissolved (do not shake)
  • Label vial with reconstitution date and concentration; protect from light
  • Refrigerate after reconstitution (commonly 2–8 °C) unless protocol states otherwise
  • Avoid repeated freeze–thaw cycles
  • Bacteriostatic Water for Injection contains benzyl alcohol preservative (multi-dose); follow protocol for beyond-use (many peptide protocols use ~28 days after mixing)
  • Avoid benzyl-alcohol-containing diluents in neonates/infants (safety warning for benzyl alcohol)